The Total RNA Extraction Mini Kit (Plant Tissues) provides an efficient method for purifying total RNA from plant tissue and cells. Samples are ground in liquid nitrogen and then filtered to remove cell debris. In the presence of a binding buffer and chaotropic salt, total RNA in the lysate binds to the glass fiber matrix of the spin column. Optional DNase treatments can be followed to remove unwanted DNA residue. Once contaminants have been removed using the Wash Buffer (containing ethanol), the purified total RNA is eluted by RNase-free water. The procedure does not require phenol extraction or alcohol precipitation and can be completed within 30 minutes.
- Sample :
up to 100 mg (fresh plant tissues)
up to 25 mg (dry plant tissues) - Format :
spin column (centrifuge) - Yield :
5-30 μg - Operation time :
within 30 minutes
RT-PCR, Northern blotting, real-time PCR
The quality of the Total RNA Extraction Mini Kit (Plant Tissue) is tested on a lot-to-lot basis by isolating total RNA from 25 mg of young leaf sample. The Purified RNA is quantified with a spectrophotometer and checked by electrophoresis.
| RB Buffer | 30 ml |
| PRB Buffer | 30 ml |
| W1 Buffer | 30 ml |
| Wash Buffer | 12.5 ml |
| RNase-Free Water | 6 ml |
| Filter Column | 50 pcs |
| RB Column | 50 pcs |
| 2 ml Collection Tube | 100 pcs |
| RB Buffer | 60 ml |
| PRB Buffer | 60 ml |
| W1 Buffer | 50 ml |
| Wash Buffer | 25 ml |
| RNase-Free Water | 6 ml |
| Filter Column | 100 pcs |
| RB Column | 100 pcs |
| 2 ml Collection Tube | 300 pcs |
| RB Buffer | 160 ml |
| PRB Buffer | 160 ml |
| W1 Buffer | 130 ml |
| Wash Buffer | 25 ml |
| RNase-Free Water | 50 ml × 2 |
| Filter Column | 300 pcs |
| RB Column | 300 pcs |
| 2 ml Collection Tube | 600 pcs |
