The miRNA Isolation Kit was designed for purifying micro RNAs (miRNAs) and other small cellular RNAs from animal tissues, paraffin-embedded tissues, cultured cells and fresh blood. Purification of miRNAs allows for research into biologically significant pathways for gene regulation. Standard protocols for isolating total RNA and mRNA are not optimized for isolation of small RNA molecules and result in the loss of substantial amounts of miRNAs and other small RNA. In addition, removal of the predominantly lager RNAs is required for accurate analysis of miRNA expression by qPCR or microarray analysis. This kit ensures purification of small RNA with minimal contamination from large RNA molecules or genomic DNA. In the presence of chaotropic salt, and various ethanol concentrations in the solvent, RNA molecules (of various sizes) are selectively bound by the glass fiber matrix.
- Sample :
100 mg of tissues
up to 25 mg of paraffin-embedded tissues
cultured cells (up to 1 x 106)
up to 200 µl of fresh blood - Format :
spin column (centrifuge) - Operation :
within 30 minutes - Yield :
high yield of miRNA and other small RNA - Efficiency :
efficient removal of larger RNA for sensitive downstream applications
Northern blot analysis, quantitative, real-time RT-PCR, microarray analysis
Samples are analyzed by gel electrophoresis. DNA fragments greater than 40 bp from the 10 bp DNA Ladder must not be detectable in purified samples
| Lysis Buffer | 25 ml |
| Mi Buffer | 3 ml |
| Wash Buffer | 1.25 ml |
| Release Buffer | 6 ml |
| RNA Column | 200 pcs |
| 2 ml Collection Tube | 200 pcs |
