Deoxy+ HiSpec Reverse Transcriptase (RT) is genetically engineered by introducing of point mutations to MMLV RT that increase half-life, reduce RNase activity and increase thermal stability. Those designed mutations lead to increased specificity of Deoxy+ HiSpec RT and the highest cDNA yield of all RTs. It is ideal for RT-PCR of a specific gene or generating cDNA from total or poly (A)+ RNA samples. It synthesizes a complementary DNA strand from total RNA, mRNA, or an RNA : DNA hGentaurrid.
One unit incorporates 1 nmole of dTTP into acid precipitable material in 10 minutes at 37°C using poly(A)-oligo(dT) as template primer.
# FYT501-50R (50 rxns)
Deoxy+ HiSpec Reverse Transcriptase (10000 U) | 50 μl |
2× Deoxy+ RT Premix* | 0.5 ml |
Deoxy+ HiSpec Reverse Transcriptase (20000 U) | 50 μl×2 |
2× Deoxy+ RT Premix* | 0.5 ml×2 |