The performance of Deoxy+ OneStep RT-PCR Kit is tested in an RT reaction using human embryonic kidney cell lysate with primer d(T)18. The sensitivity of the kit is verified by the detection of B2M transcript after 35 cycles. The length of cDNA achieved is verified by detection of a 248 bp by DNA agarose gel and DNA sequencing.
# FYT503-50P (50 preps)
2× Deoxy+ OneStep RT PCR Premix | 0.625ml |
Sterilized ddH2O | 1 ml |
2× Deoxy+ OneStep RT PCR Premix | 0.625 ml× 2 |
Sterilized ddH2O | 1 ml×2 |