Gentaur Direct PCR Kits for Blood/Plants are formulated to perform PCR directly from whole blood or plant leaf tissues with no prior DNA extraction. The Direct PCR for Blood can process whole blood (or serum) preserved in EDTA, citrate or heparin or stored on various types of filter papers. Plant tissues even with high polyphenolic compounds are all suitable for Direct PCR for Plants. The Kits employs our especial mixes of DNA polymerases and reagents allowing higher resistance to inhibitors found in blood and plants, respectively.
The Blood Direct PCR kit has been optimized to give excellent results with blood originated from many animals. The recommended blood concentration is 1−10 % and the recommended plant tissue size is about 2 mm². Gentaur Direct PCR Kits includes a pair of universal control primers that are compatible with a number of mammalian and plant species, respectively .
The performances of Gentaur Direct PCR kits are tested in PCR reaction using human blood and with universal primer sets. The sensitivities of the kits are verified by the detection of specific sizes of products by DNA agarose gel and DNA sequencing.
Direct PCR Kit for Blood
2× Premix | 1.25 ml |
HiFi DNA Polymerase | 50 μl |
Enhancer P1 | 200 μl |
Enhancer P2 | 200 μl |
Universal Primer Set | 100 μl |
2× Premix | 1.25 ml |
HiFi DNA Polymerase | 50 μl |
Enhancer P1 | 200 μl |
Enhancer P2 | 200 μl |
Universal Primer Set | 100 μl |