YEA-His Spin Kit
Description

YEA-His Spin Kit is specially designed to make protein purification as easy as DNA Purification. Pre-packed columns with high quality resin and simple protocols speed up your work flow and make protein purifications more reliable. From single sample to high throughput protein purification, you can achieve results faster. The ready-to-use column contains immobilized metalions affinity chromatography resin (YealoseR) .
Features

Histidine tagged protein purification has never been easier. The columns contain pre-packed Ni2+ Yealose resin which have a high specific binding affinity to 6× histidine residues. Simple bind, wash and elute high-purity protein with these ready-to-use columns. YEA-His Spin Kit gives exceptionaly high-yield recovery.
Applications

  • Histidine tagged protein purification
  • Multi-TOF analysis
  • Covalent modification
Quality Control

Each lot of YEA-His kit is tested against preset specifications to ensure consistent quality.
Protocol

Specification
Store at

 

# FYP102-20P (20 preps)
His Yealose 1 ml x 4
Enzyme Mix B 1 ml
Equilibration buffer 25 ml
Washing buffer   40 ml
Elution buffer 20 ml
Spin Column/Collection tube 20 pcs
# FYP102-52P (52 preps)
His Yealose 1 ml x 11
Enzyme Mix B 2.6 ml  
Equilibration buffer 60 ml  
Washing buffer 90 ml  
Elution buffer 60 ml  
Spin Column/Collection tube 52 pcs
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