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Cloning, Mutagenesis and Lentiviral Packaging


 

Receive superior shRNA-mir designs in your choice of vector

shRNA-mir found through FETCH my gene can be transferred to the lentiviral vector (pZIP) or other mir-adapted vectors.  Use the gene search to select shRNA-mir and follow the link for lentiviral options to have them subcloned into pZIP.

Cloning workflow includes:

  • Transfer of the shRNA-mir in to lentiviral vector
  • Full insert sequence validation
  • Transformation in to E. coli
  • Delivery as bacterial glycerol stock, plasmid DNA or lentiviral particles
Turnaround time is ~2 weeks.

 

 
Catalog Number Format* Components More information
TLHS9600 Target Gene Set 3-4 shRNA-mir plus non-targeting control (Human)
TLHK9600 Starter Kit 3-4 shRNA-mir plus  positive and non-targeting controls (Human)
TLMS9600 Target Gene Set 3-4 shRNA-mir plus non-targeting control (Mouse)
TLMK9600 Starter Kit 3-4 shRNA-mir plus positive and non-targeting controls (Mouse)
*shRNA-mir provided as bacterial glycerol stock clones.