GENTAUR - Research solutions for the academic, biotechnology and pharmaceutical industries
aCella™ - cAMP |
||||||
Key Benefits: | ||||||
|
||||||
Introduction to aCella - cAMP | ||||||
Cyclic AMP (cAMP) is one of the most important secondary messengers and a key intracellular regulator. Discovered by Sutherland and Rall in 1957, it functions as a mediator of activity for a number of hormones, including epinephrine, glucagon, and ACTH. Cyclic AMP is produced by the enzyme adenylate cyclase, and the enzyme is activated by the hormones glucagon and adrenaline and by G protein. cAMP decomposition into AMP is catalyzed by the enzyme phosphodiesterease. Other biological actions of cAMP include regulation of innate immune functioning, axon regeneration, cancer and inflammation. |
||||||
Assay Principle: | ||||||
The aCella – cAMP Chemilumnescent Immunoassay kit is designed to quantitatively measure cAMP present in cell lysates, plasma, urine, saliva, tissue and culture media samples. The supplied Sample Diluent will lyse cells, stabilize cAMP and stop phopshodiesterease activity. A cAMP standard is provided to generate a standard curve for the assay. The supplied Plate Primer solution is added to the wells of a coated white microtiter plate, followed by standards or diluted samples. A cAMP-peroxidase conjugate is then added to the wells.
|