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Fluoro Sarcosine
Fluorescent Sarcosine Detection Kit
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| Key Benefits: |
- Easy to Use - No Wash Assay.
- Detection of Sarcosine in cells, serum, tissue extracts..
- Easy to Use 96-well Fluorescent Plate reader readout.
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| Introduction to Fluoro Sarcosine |
Sarcosine is natural amino acid that is an important intermediate in the metabolism of choline. Sarcosine is an important component of proteins and plays a significant role in metabolic processes of living cells as a source of serine, creatine, purines and glutathione etc. Sarcosine is present in food sources like legumes, eggs, ham, turkey etc. It is used in a variety of industrial applications such as manufacturing of tooth paste and biodegradable surfactants. Sarcosine was recently reported to activate prostate cancer cells and as a possible marker for prostate cancer progression to metastasis(1). It is also used in adjunctive treatment for Schizophrenia (2) and depression (3).
Gentaur’s Fluoro Sarcosine assay provides a reliable, sensitive fluorimetric method for the quantification of sarcosine in biological samples.
The Fluoro Sarcosine detection kit utilizes a non-fluorescent detection reagent, which is reduced via an enzyme-coupled reaction in the presence of sarcosine. A sarcosine standard curve is generated to quantify sarcosine in the samples. |
| Reaction |
Sarcosine + non-fluorescent detection reagent+ Enzyme Mix -----> fluorescent analog
Excitation: 530-570nm and Emission at 590-600 |
Table 1: We conducted spike and recovery experiments to estimate % recovery of sarcosine. Serum was spiked with sarcosine with the concentrations mentioned in the table above. The samples were processed as described in section IX of the protocol.
Table 2: We conducted spike and recovery experiments to estimate % recovery of sarcosine. Cell lysates were spiked with sarcosine with the concentrations mentioned in the table above. The samples were processed as described in section VIII: Mammalian Cell Preparation.
Table 3: Serum samples were diluted 1:5 in standard curve diluent. Jurkat and Daudi cells were prepared as described in the protocol. After the final wash cells were adjusted to 1X106 cells/mL in standard curve diluent. Sarcosine was measured as described in the protocol.
Figure 1. Sarcosine standard curve was generated as described in the protocol. R2= 0.998
| Kit contents: |
| 1. |
Enzyme Mix (Part# 6024): 1 vial. Upon arrival store at -20°C. |
| 2. |
Standard Curve Diluent (Part# 3056): 40 mL. Upon arrival store at 2-8°C. Ready to use. |
| 3. |
Sarcosine Detection Reagent (Part# 4023): 1 vial. Upon arrival store at 2-8°C. |
| 4. |
Sarcosine Standard (Part# 7021): 1 vial. Upon arrival store at 2-8°C. |
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