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GENTAUR - Research solutions for the academic, biotechnology and pharmaceutical industries


Fluoro H2O2™ 
Fluorescent Hydrogen peroxide/Peroxidase Detection Kit
Key Benefits:
  • Quick 10 minute assay.
  • Can monitor multiple time points to follow kinetics.
  • Dual mode, can detect H202 or peroxidase activity.
  • One-step, no wash assay.
  • Adaptable for High Throughput format.
  • Non-destructive cell based assay allows monitoring of additional parameters.
  • Applications - Fluorescent Plate Reader.
Assay Principle:

The Fluoro H202 detection kit utilizes a non-fluorescent detection reagent, to detect H202. H202 oxidizes the detection reagent in a 1:1 stoichiometry to produce a fluorescent product resorufin. This oxidation is catalyzed by Peroxidase in a homogeneous no wash assay system.

The detection reagent can be utilized to detect H202 release from cells or enzyme coupled reactions (1-7)
Reaction:

peroxidase H202 + detection reagent (non-fluorescent) ---------> Resorufin (fluorescent) Excitation: 530-571nm Emmission: 590-600nm
Flouro


Figure 1. Hydrogen Peroxide titrated in 1X reaction Buffer and measured using the Fluoro H202 Kit.


Product Catalog # Size
Fluoroscent Hydrogen peroxide/Peroxidase Detection Kit FLOH 100-3 500