Stem Cell Characterization


Stem Cell Characterization

We offer multiple products for embryonic stem cell (ESC) and induced pluripotent stem cell (iPSC) characterization. See below for our pluripotency protein marker kits, pluripotency mRNA marker kits, stem cell gene arrays and other individual components for our characterization kits.

Pluripotency Markers (Protein) :

We offer pluripotency protein markers and ready-to-use embryonic stem cell, induced pluripotent stem cell (ESC/iPSC) characterization kits that contain all the antibodies, buffers and solutions necessary to perform immunocytochemical and enzyme activity analysis.

ESC/iPSC Characterization Kit (Antibody Marker Kit)

Gentaur’s mouse embryonic stem cell, induced pluripotent stem cell (mES/iPSC) characterization kit is designed for users to examine the pluripotent status of mouse embryonic stem cells/induced pluripotent stem cells (mES/iPSC) by analyzing marker protein expression. With a standard cell culture and immunofluorescence staining protocol, our optimized, ready-to-use kit provides a fast, convenient, sensitive method for detecting the pluripotent status of mES/iPS cells. Three commonly used marker antibodies, anti-Oct4, anti-Sox2, and anti-SSEA-1, are included in the kit with optimized concentration for immunofluorescence staining. Oct4 and Sox2 are transcription factors highly expressed in undifferentiated ESC and embryonic germ cells (EGC). SSEA-1 is a globoseries carbohydrate antigen present on the surface of murine ESC, but not human ESC. In addition, Gentaur’s mouse embryonic stem cell/induced pluripotent stem cell (mES/iPSC) characterization kit provides a rapid and sensitive alkaline phosphatase (AP) activity test. AP is a stem cell membrane marker and elevated expression of AP is associated with the pluripotent status. See below for more information on our embryonic stem cell and induced pluripotent stem cell pluripotency RT-PCR kits for mice and humans.

Gentaur’s Mouse Embryonic Stem Cell/Induced Pluripotent Stem Cell (mES/IPSC) Characterization Kit Includes:

  1. Easy-to-use primary and secondary antibodies with well-established pluripotency markers to confirm the pluripotency of human or mouse ESC/iPSC lines.
  2. Fixation, Permeabilization, Blocking, and Mounting Reagents
  3. DNA counterstain
  4. Alkaline Phosphatase activity assay

 Sophisticated Marker Selection:

Human: OCT4, SOX2, SSEA4, TRA-1-60, TRA-1-81

Mouse: OCT4, SOX2, SSEA1

 

High Sensitivity:  Partially differentiated hiPSC clones can be detected.

Convinient: The ready-to-user kit contains all the buffers and solutions needed to perform immunocytochemical and enzyme activity analysis.

 

Pluripotency mRNA Markers :

RT-PCR kit to confirm the pluripotency of human or mouse embryonic stem cell and induced pluripotent stem cell (ES/iPS) lines with well established pluripotency markers.

ES/iPS Cell Pluripotency RT-PCR Kit

Gentaur provides a embryonic stem cell and induced pluripotent stem cell (ESC/iPSC) pluripotency RT-PCR kits for mice and humans.

Two distinguishing characteristics of stem cells are their capacity for self-renewal and their ability to maintain pluripotency. The expression of stem cell specific genes has been generally accepted as one method to characterize the pluripotency of a stem cell line. The most commonly used biomarkers to determine pluripotency of mouse embryonic stem/induced pluripotent stem (mES/iPS) cell lines are Nanog, Oct4, Sox2, Tbx3, Esrrb and Tcl1 Nanog, Oct4 and Sox2 are transcription factors that are highly expressed in undifferentiated ESC and embryonic germ cells (EGC). Tbx3 is found to improve the competency of mouse induced pluripotent stem cells (iPSC). The expression of Esrrb and Tcl1 is significantly different between pluripotent and differentiated cells. The mouse embryonic stem/induced pluripotent stem cell (ESC/iPSC) pluripotency RT-PCR kit includes primer sets to detect all six pluripotency markers. A set of Actin primers is included as a positive control.

Stem Cell Array :

Stem Cell related Gene Arrays (miRNA, TF, cDNA and gene expression).

Stem cell related gene arrays (miRNA, TF, cDNA and gene expression).

Stem cells can differentiate into all of the specialized embryonic tissues during embryo development. In adult organisms, stem cells and progenitor cells act as a repair system for the body, replenishing specialized cells in order to maintain the normal turnover of regenerative organs such as blood, skin, or intestinal tissues. Recent studies suggest that maintenance and differentiation of stem cells are regulated by microRNAs (miRNA). miRNAs are small single-stranded RNA molecules (~20 nt long) that play an important role in gene regulation and cell differentiation. miRNAs are found to be differentially expressed in stem cells, such as brain-specific miR-124a and miR-9 molecules during neural lineage differentiation. In addition, miRNAs are also reported to regulate cancer stem cells. We have developed a miRNA array to specifically target 69 miRNAs that have been shown in research literature to be associated with stem cell differentiation and maintenance. The array can be used for human, mouse, and rat samples. Profiling the expression of these miRNAs will help to reveal the functions of miRNAs in stem cells.

Components :

We offer many individual components including solutions and secondary antibodies for our stem cell characterization kit.

We offer many individual components including solutions and secondary antibodies for our stem cell characterization kit.