Human iPSCs

Gentaur offers Human induced pluripotent stem cells (iPSCs). Human induced pluripotent stem (iPS) cell lines are generated by retrovirus vectors or episomal expression.

We offer a human iPS (induced pluripotent stem) cell line that was derived from human foreskin fibroblasts (HFFs) by retroviral expression of OCT4, SOX2, KLF4, and c-MYC genes. The cells were derived using morphological selection criteria and without the use of fluorescent marker or drug selection. When cultured under standard human ES cell culture conditions, the morphology of human iPS cells is identical to that of human ES cells. The cells also express the pluripotency markers SSEA-3 and Nanog and demonstrate strong endogenous alkaline phosphatase. These high-viability, low-passage iPS cells have been pre-adapted to serum-free, feeder-free culture conditions.

Additionally we offer a footprint-free human iPS (induced pluripotent stem) cell line was derived from human foreskin fibroblasts (HFFs) by ectopic expression of OCT4, SOX2, KLF4, and c-MYC genes using episomal plasmids. The cells were derived using morphological selection criteria and without the use of fluorescent marker or drug selection. When cultured under standard human ES cell culture conditions, the morphology of human iPS cells is identical to that of human ES cells. The cells also express the pluripotency markers SSEA-3 and Nanog and demonstrate strong endogenous alkaline phosphatase. These high-viability, low-passage iPS cells have been pre-adapted to serum-free, feeder-free culture conditions.