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Primer Premier

A comprehensive PCR primer design software

Comprehensive Design Support
Primer Premier's search algorithm finds optimal PCR, multiplex and SNP genotyping primers with the most accurate melting temperature using the nearest neighbor algorithm. Primers are screened for secondary structures, dimers, hairpins, homologies and physical properties before reporting the best ones for your sequence, in a ranked order. Load the gene of interest from NCBI, select a search range, sit back and
let Primer Premier pick the best possible primers for you.
Automatic Homology & Template Structure Avoidance
Primer Premier automatically interprets the BLAST search results and utilizes a proprietary algorithm to check for possible secondary structures. Homologies and structures are avoided for designing highly specific and efficient primers.
Multiplex Assays & SNP Genotyping
For a multiplex experiment, Primer Premier enables you to launch a primer search in batch mode and then checks the cross reactivity of the primers designed, thereby reducing false priming. For SNP genotyping assays, SNP flanking primers are designed.
Versatile Primer Design & Analysis
Using the manual primer search option, a user can click any nucleotide on the template sequence to get primers at the location of their choice. Primer Premier instantly analyzes the primer properties. The program
also evaluates the properties of pre-designed primers and ranks them for you to adjudge how far the primers are from their desired target values. This feature is especially useful if you are working with published or
pre-designed well proven primers. For any given primer, it even designs a compatible forward or reverse primer.

To activate & evaluate, follow these steps
- Install Primer Premier from the website or the CD
- Launch and click Evaluate on the first window that opens
- Enter the evaluation key requested from us

Cat.Number: PP3

PRICE: $1 085,00