Primer Premier
A comprehensive PCR primer design software
Comprehensive Design Support
Primer Premier's search algorithm finds
optimal PCR, multiplex and SNP
genotyping primers with the most
accurate melting temperature using the
nearest neighbor algorithm. Primers are
screened for secondary structures,
dimers, hairpins, homologies and
physical properties before reporting the
best ones for your sequence, in a ranked
order. Load the gene of interest from
NCBI, select a search range, sit back and
let Primer Premier pick the best possible
primers for you.
Automatic Homology & Template Structure Avoidance
Primer Premier automatically interprets the BLAST search results and utilizes a proprietary
algorithm to check for possible secondary structures. Homologies and structures are
avoided for designing highly specific and efficient primers.
Multiplex Assays & SNP Genotyping
For a multiplex experiment, Primer Premier
enables you to launch a primer search in batch
mode and then checks the cross reactivity of the
primers designed, thereby reducing false priming.
For SNP genotyping assays, SNP flanking primers
are designed.
Versatile Primer Design & Analysis
Using the manual primer search option, a user can
click any nucleotide on the template sequence to get
primers at the location of their choice. Primer Premier
instantly analyzes the primer properties. The program
also evaluates the properties of pre-designed primers
and ranks them for you to adjudge how far the primers
are from their desired target values. This feature is
especially useful if you are working with published or
pre-designed well proven primers. For any given
primer, it even designs a compatible forward or
reverse primer.
To activate & evaluate, follow these steps
- Install Primer Premier from the website or the CD
- Launch and click Evaluate on the first window that opens
- Enter the evaluation key requested from us
Cat.Number: PP3
PRICE: $1 085,00