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Oligo design tool for xMAP® based multiplex systems

PrimerPlex is an efficient and sophisticated tool for designing oligos for multiplex assays. Multiplex assays facilitate amplification of multiple targets in a single reaction vessel, reducing both, the time and cost of
experimentation. PrimerPlex designs oligos for multiplex PCR assays and for suspension array based systems such as MagPix, Luminex 200 & FlexMAP 3D.

Standard Multiplex PCR

Multiplex assays facilitate amplification of multiple targets in a single reaction vessel, reducing both, the time and cost of experimentation. Multiplex PCR applications now include pathogen identification, gender screening, linkage analysis, forensic studies, template quantitation, and genetic disease diagnosis. The algorithms runs through hundreds of primer sets to find primers that not only work efficiently for the sequence but also do not cross hybridize with any other sequence in the pool, or interact with other primer sets of other sequences to assure high signal strength. The primers are designed under uniform reaction conditions to enable amplification under standard experimental conditions. Primers for standard multiplex PCR are designed for different amplicon lengths, different enough to form discernible bands when visualized on a gel.
Evaluate Primers and Probes

PrimerPlex can use pre-designed well proven oligos to build a multiplex set. After specifying the oligos, their properties are analyzed and the user is alerted for any deviations so found. For the sequences where pre-designed oligos are not specified, PrimerPlex designs them, checks them for multiplexing and highlights compatibility issues with the pre-existing ones. The user can then decide to accept the design or create a separate pool, giving complete control in the hands of user.
Direct Hybridization Assays
PrimerPlex designs specific capture probes for direct hybridization assays. Their specificity is assured by avoiding significant cross homologies identified by automatically interpreting BLAST search results. For genotyping assays, the probes are designed such that the mutations are at their centers. The innovative and proprietary algorithm analyzes each one of these probes for their suitability in a multiplex reaction. The probes are designed by minimizing Tm mismatches and are screened for cross and self dimers, assuring strong signal strength. This functionality makes PrimerPlex the only software product capable of designing probes for 150 individual targets in a single reaction vessel. Also included is the ability to design compatible primer pairs for amplifying the template.
Allele Specific Primer Extension (ASPE) Assays
PrimerPlex designs highly specific primers for the novel Allele Specific Primer Extension (ASPE) assays. The Allele Specific Primer Extension (ASPE) primers are designed such that the mutation lies at their 3' end. The primers are analyzed for their suitability in a multiplex reaction as described above. ASPE assays can be used to detect sequence variations in a large number of samples for applications such as disease diagnostics, drug discovery, bacterial identification & forensic analysis.

To activate & evaluate, follow these steps
- Install PrimerPlex from the website or the CD
- Launch and click Evaluate on the first window that opens
- Enter the evaluation key requested from us

Cat.Number: P21

PRICE: $2 685,00