PrimerPlex
Oligo design tool for xMAP® based multiplex systems
PrimerPlex is an efficient and
sophisticated tool for designing oligos
for multiplex assays. Multiplex assays
facilitate amplification of multiple targets
in a single reaction vessel, reducing
both, the time and cost of
experimentation. PrimerPlex designs
oligos for multiplex PCR assays and for
suspension array based systems such
as MagPix, Luminex 200 & FlexMAP
3D.
Standard Multiplex PCR
Multiplex assays facilitate amplification
of multiple targets in a single reaction
vessel, reducing both, the time and cost of
experimentation. Multiplex PCR applications now include
pathogen identification, gender screening, linkage analysis, forensic studies, template quantitation,
and genetic disease diagnosis. The algorithms runs through hundreds of primer sets to find
primers that not only work efficiently for the sequence but also do not cross hybridize with any
other sequence in the pool, or interact with other primer sets of other sequences to assure high
signal strength. The primers are designed under uniform reaction conditions to enable amplification
under standard experimental conditions. Primers for standard multiplex PCR are designed for
different amplicon lengths, different enough to form discernible bands when visualized on a gel.
Evaluate Primers and Probes
PrimerPlex can use pre-designed well proven oligos to build a multiplex set. After specifying the
oligos, their properties are analyzed and the user is alerted for any deviations so found. For the
sequences where pre-designed oligos are not specified, PrimerPlex designs them, checks them
for multiplexing and highlights compatibility issues with the pre-existing ones. The user can then
decide to accept the design or create a separate pool, giving complete control in the hands of user.
Direct Hybridization Assays
PrimerPlex designs specific capture probes for direct hybridization assays. Their specificity is
assured by avoiding significant cross homologies identified by automatically interpreting BLAST
search results. For genotyping assays, the probes are designed such that the mutations are at
their centers. The innovative and proprietary algorithm analyzes each one of these probes for their
suitability in a multiplex reaction. The probes are designed by minimizing Tm mismatches and are
screened for cross and self dimers, assuring strong signal strength. This functionality makes
PrimerPlex the only software product capable of designing probes for 150 individual targets in a
single reaction vessel. Also included is the ability to design compatible primer pairs for amplifying
the template.
Allele Specific Primer Extension (ASPE) Assays
PrimerPlex designs highly specific primers for the novel Allele Specific Primer Extension (ASPE)
assays. The Allele Specific Primer Extension (ASPE) primers are designed such that the mutation
lies at their 3' end. The primers are analyzed for their suitability in a multiplex reaction as described
above. ASPE assays can be used to detect sequence variations in a large number of samples for
applications such as disease diagnostics, drug discovery, bacterial identification & forensic
analysis.
- Install PrimerPlex from the website or the CD
- Launch and click Evaluate on the first window that opens
- Enter the evaluation key requested from us
Cat.Number: P21
PRICE: $2 685,00