Beacon Designer™
Design SYBR ® Green primers, primers for HRMA, TaqMan spiked TaqMan ® probes, Scorpions ® probes, MethyLight TaqMan ® probes, LNA ® , FRET probes or molecular beacons for robust amplification and fluorescence in real time PCR
Optimal SYBR ® Green Primer Design
Use Beacon Designer™ to design highly
specific and efficient SYBR
®
Green primers.
Specificity is assured by avoiding significant
cross homologies identified by automatically
interpreting BLAST search results. To assure
efficient hybridization and high yield, the
program avoids template secondary
structures for locating primers.
High Resolution Melt Analysis
(HRMA) Primer Design
Beacon Designer™ offers a
comprehensive solution for mutation
detection. High Resolution Melt
Analysis (HRMA) is a more cost-
effective method than probe-based
genotyping analysis. The program
employs proprietary algorithms that
enable designing the best primers for
detecting SNPs, MNPs, DIPs and Mixed type mutations.
Design Multiplex Assays
Beacon Designer™ uses innovative proprietary algorithms to design optimal primer-probe sets
for single tube multiplex assays. You can choose the set best suited for your experimental
needs from a list of alternates presented in variously sortable order. If you want to incorporate
well-proven sets for some of the templates, or include reference or housekeeping genes for
normalization purposes in the assay, Beacon Designer™ is equipped to fully support your
needs.
Dual Labeled Probe Design
Beacon Designer™ offers comprehensive support for designing TaqMan ® , molecular beacon, Scorpions ® and FRET assays for studying differential gene expression or detecting SNPs using single template or multiplex reactions. You can even confirm SYBR ® Green assay results by designing probes compatible with previously designed primer pairs for any of these techniques. Or simply evaluate partially or fully predesigned assays using instant analysis capabilities of Beacon Designer™.
LNA™, MethyLight, NASBA ® & Scorpions ® Assays
Design the increasingly popular Locked Nucleic Acid (LNA™) substituted TaqMan
®
probes,
which are more stable than standard probes. TaqMan
®
probes can also be designed for
MethyLight assays. MethyLight is a high throughput assay used to discriminate between
methylated and unmethylated DNA. Nucleic acid sequence-based amplification (NASBA®
) is a
primer-dependent technology that can be used for the continuous amplification of nucleic acids
in a single mixture at one temperature. You can now design molecular beacons for NASBA
assays for single template or multiplex reactions. Design Scorpions
®
to achieve
sequence-specific priming and PCR product detection using a single oligonucleotide.
®
To activate & evaluate, follow these steps
- Install Beacon Designer
TM
from the website or the CD
- Launch and click Evaluate on the first window that opens
- Enter the evaluation key requested from us
Cat.Number: BD3
PRICE: $2 585,00