• Home
• Suppliers

Single-reaction labeling kit with biotinylation reproducibility
The ChromaLink™ One-Shot Antibody Biotinylation Kit provides convenient, measurable biotinylation of 100 µg of antibody. The kit contains ChromaLink™ Biotin, which incorporates a UV-traceable chromophore, based on Solulink’s proprietary linking technology, to enable reproducibility in your biotinylation process. Now you can measure the degree of biotinylation in minutes, not hours, without the standard curves required for HABA/avidin and fluoro-reporter assays. With a simple and direct UV scan (A₂₈₀ and A₃₅₄), you can quantify biotin incorporation and ensure reproducible production of consistent batches.

The ChromaLink™ One-Shot Antibody Biotinylation Kit includes everything you need to biotinylate your antibody:  buffers, reagents, spin columns, and easy-to-follow protocols. With the ChromaLink™ One-Shot Antibody Biotinylation Kit, you can biotinylate and purify 100 µg of antibody in about 2.5 hours. The resulting biotinylated antibody is ready for binding to streptavidin. We recommend our NanoLink™ Streptavidin Magnetic Beads (Cat. No. M-1002-010) with the highest biotin-binding capacity available for high-sensitivity ELISA, western blot, IHC, or immunoassay development.

ChromaLink™ One-Shot Antibody Biotinylation Kit Advantages

• Reproducible Production – UV-traceable chromophore permits nondestructive, rapid A₂₈₀/A₃₅₄ quantification of incorporated biotin, enabling consistency and reproducibility
• Extended PEG3 Spacer – Helps reduce aggregation, minimizes steric hindrance, and enhances solubility
• One-shot – All components sized to quickly label 100 µg of antibody
• Convenient – Kit contains buffers, spin columns, and calculator to determine MSR

1. Sweetwyne MT, Pallero MA, Lu A, Van Duyn Graham L, Murphy-Ullrich JE. The Calreticulin-Binding Sequence of Thrombospondin 1 Regulates Collagen Expression and Organization During Tissue Remodeling. American Journal of Pathology 2010;177(4):1710-24.
2. Sharma RK, Schabowsky RH, Srivastava AK, Elpek KG, Madireddi S, Zhao H, Zhong Z, Miller RW, MacLeod KJ, Yolcu ES, Shirwan H. 4-1BB Ligand as an Effective Multifunctional Immunomodulator and Antigen Delivery Vehicle for the Development of Therapeutic Cancer Vaccines. Cancer research 2010;70(10):3945.
3. Dirksen A, Yegneswaran S, and Dawson PE. Bisaryl Hydrazones as Exchangeable Biocompatible Linkers.Angew. Chem. Int. Ed. 2010,49,2023–2027.
4. Srinivasan B, Li Y, Jing Y, et al. A detection system based on giant magnetoresistive sensors and high-moment magnetic nanoparticles demonstrates zeptomole sensitivity: potential for personalized medicine.Angew. Chem. Int. Ed. Engl. 2009;48(15):2764-2767.
5. Ma Z, Li Q, An H, Pankonin MS, Wang J, Loeb JA. Targeting Human Epidermal Growth Factor Receptor Signaling with the Neuregulin's Heparin-binding Domain. Journal of Biological Chemistry 2009;284(46):32108.